ALAS1 antibody (AA 480-530)

Catalog No. ABIN2470088

Product Details anti-ALAS1 Antibody

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Binding Specificity: AA 480-530

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Protein G Column

Immunogen: ALAS1 antibody was raised against amino acids 480 - 530 of ALAS1 (Human).

Isotype: IgG

Application Details

Application Notes: ALAS1 antibody can be used in ELISA, and immunohistochemistry starting at 5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store ALAS1 antibody at 4 °C for short term applications. For long term storage, aliquot and -20 °C.

Target Details for ALAS1

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Alternative Name: ALAS1 (ALAS1 Products)

Background: ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzymecatalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5 phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.

Gene ID: 211

UniProt: P13196

Pathways: Regulation of Lipid Metabolism by PPARalpha