Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
Recognizes human mast cell tryptase, both alpha and beta isoforms. Is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.
Purification
Protein G Column
Immunogen
Tryptase monoclonal antibody was raised against lug tissue Tryptase (Human).
TPSAB1
Reactivity: Human, Mouse, Rat
WB, IHC, ELISA, ICC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Tryptase antibody can be used in ELISA, and immunohistochemistry starting at 10 μg/mL. This product requires protein digestion pre-treatment of paraffin sections e.g. pronase. Results may also be obtained with this produst using HIER. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4, 0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
As with all antibodies avoid freeze/thaw cycles. Store undiluted.