ADP-Ribosylation Factor 1 (ARF1) antibody

Antigen: ADP-Ribosylation Factor 1 (ARF1)

Synonyms: ARD1, ARFD1, RNF46, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, MGC72830, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, arf2, MGC52628, arf-1, MGC52573, TRIM23, ADP-RIBOSYLATION FACTOR, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION FACTOR 1A, ATARF, ATARF1, ATARFA1A, F28C11.12, arf1, A2MP, DKFZp564M0264, AI788669, AW496366, MGC102530, ARFGAP1, FLJ45618, ARF1GAP, MGC39924, HRIHFB2281, Arfgap1, 0610009H19Rik, 1810004P07Rik, 1810035F16Rik, 9130416J18Rik, Arf1gap, AI115377, Arf GAP1, MGC53624, arf6, MGC76053, MGC80156, arfgap1, MGC83573, MGC79593, ARFGAP3, ARF1A1C, T8I13.1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Easter lily (Lilium longiflorum), Tobacco (Nicotiana tabacum), Chlamydomonas reinhardtii (C. reinhardtii)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN249383

Additional Information

Immunogen: recombinant GST fusion of full length of Arabidopsis thaliana ARF1 At2g47170

Format: Lyophilized

Description: The ARF1 protein is localized to the Golgi apparatus and has a central role inintra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotideexchange cycle and it is an important regulator of cellular trafficking.

Specificity: Predicted reactivity: dicots including B. juncea, B.napus, C.annum, D.carota, E.guineensis,G.max,H.orientalis, M.truncatula, N.benthamina, S.tuberosum monocots includingO.sativa, moss Physcomitrella patens.

Synonyms: ARD1, ARFD1, RNF46, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, MGC72830, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, arf2, MGC52628, arf-1, MGC52573, TRIM23, ADP-RIBOSYLATION FACTOR, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION FACTOR 1A, ATARF, ATARF1, ATARFA1A, F28C11.12, arf1, A2MP, DKFZp564M0264, AI788669, AW496366, MGC102530, ARFGAP1, FLJ45618, ARF1GAP, MGC39924, HRIHFB2281, Arfgap1, 0610009H19Rik, 1810004P07Rik, 1810035F16Rik, 9130416J18Rik, Arf1gap, AI115377, Arf GAP1, MGC53624, arf6, MGC76053, MGC80156, arfgap1, MGC83573, MGC79593, ARFGAP3, ARF1A1C, T8I13.1

Application Details

Application Notes: Recommended Dilution 1:1000 with standard ECL (WB), 1: 1000 (IF).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Nicotiana tabacum protoplast total protein with Triton X100, 0.02% (1), Arabidopsis thaliana protoplast soluble protein (2), Arabidopsis thaliana protoplast total protein with Triton X100 0.02% (3), (50 ug of total protein extracts from cell cultures were loaded per lane)

anti-ADP-Ribosylation Factor 1 (ARF1) antibody (Image 2)

Publications

Publications: Pimpl, Movafeghi, Coughlan et al.: "In situ localization and in vitro induction of plant COPI-coated vesicles." in: The Plant cell, Vol. 12, Issue 11, pp. 2219-36, 2001 (PubMed).