PAM antibody (C-Term)

Catalog No. ABIN2498842

The Rabbit Polyclonal anti-PAM antibody (ABIN2498842) specifically detects PAM in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog and Zebrafish (Danio rerio) samples.

Quick Overview for PAM antibody (C-Term) (ABIN2498842)

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAM Antibody

Binding Specificity: C-Term

Sequence: GLNLGNFFAS RKGYSRKGFD RLSTEGSDQE KEDDGSESEE EYSAPLPALA

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 87%, Rat: 93%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against PAM. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human PAM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Alternative Name: PAM

Background: This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Alias Symbols: PAL, PHM
Protein Size: 335

Molecular Weight: 36 kDa

Gene ID: 5066

NCBI Accession: NM_000919, NP_000910

UniProt: P19021