PAM antibody (C-Term)

Catalog No. ABIN2498842

This anti-PAM antibody is a Rabbit Polyclonal antibody detecting PAM in WB. Suitable for Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog and Zebrafish (Danio rerio).

Quick Overview for PAM antibody (C-Term) (ABIN2498842)

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAM Antibody

Binding Specificity: C-Term

Sequence: GLNLGNFFAS RKGYSRKGFD RLSTEGSDQE KEDDGSESEE EYSAPLPALA

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 87%, Rat: 93%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against PAM. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human PAM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Alternative Name: PAM

Background: This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Alias Symbols: PAL, PHM
Protein Size: 335

Molecular Weight: 36 kDa

Gene ID: 5066

NCBI Accession: NM_000919, NP_000910

UniProt: P19021