Mouse anti-Human IgG2 (Fc Region) Antibody

Catalog No. ABIN2623556

Product Details

Target: IgG2

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Immunofluorescence (IF)

Specificity: Monoclonal Mouse antibody to Human IgG2, subclass specific.

Purification: Purified IgG

Immunogen: Highly purified monoclonal IgG2 isolated from human serum.

Type of Immunogen: Purified protein

Clone: NI 25-1 (HP 6207)

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IF

Usage: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG2 Molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:500 upwards. in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Target Details

Target: IgG2

Abstract: IgG2 Products

Target Type: Antibody