Myogenic Factor 5 (MYF5) antibody

Antigen: Myogenic Factor 5 (MYF5)

Synonyms: bHLHc2, Myf-5, B130010J22Rik, cb641, myf-5, MGC110509, zgc:110509, MYF5, Xmyf5, Xmyf-5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN263192

Additional Information

Alternative name: MYF5

Gene ID: 4617

Immunogen: PECNSPVWSRKSST

Format: Affinity-purified

Description: All names and symbols: MYF5 , myogenic factor 5 . Official Symbol: MYF5 . RefSeq number(s): NP_005584.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 28kDa band observed in Human Skeletal Muscle lysates (calculated MW of 28.4kDa according to NP_005584.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of 26kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN263192 (0.5µg/ml) staining of Muscle lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kuang, Kuroda, Le Grand et al.: "Asymmetric self-renewal and commitment of satellite stem cells in muscle." in: Cell, Vol. 129, Issue 5, pp. 999-1010, 2007 (PubMed).