MYF5 antibody (N-Term)

Catalog No. ABIN2779471

Cited in 1 publication. The Rabbit Polyclonal anti-MYF5 antibody (ABIN2779471) specifically detects MYF5 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Dog, Goat, Guinea Pig, Horse, Sheep and Zebrafish (Danio rerio) samples.

Quick Overview for MYF5 antibody (N-Term) (ABIN2779471)

Target: MYF5 (Myogenic Factor 5 (MYF5))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Goat, Guinea Pig, Horse, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYF5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MYF5 Antibody

Binding Specificity: N-Term

Purpose: MYF5 Antibody - N-terminal region

Sequence: ACKACKRKST TMDRRKAATM RERRRLKKVN QAFETLKRCT TTNPNQRLPK

Predicted Reactivity: Cow: 100%, Dog: 93%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against MYF5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MYF5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8105953

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MYF5 antibody (ABIN2779471)

Yang, Liu, Mannix, Xu, Li, Ma, Ingber, Allen, Wang: "Mononuclear cells from dedifferentiation of mouse myotubes display remarkable regenerative capability." in: Stem cells (Dayton, Ohio), Vol. 32, Issue 9, pp. 2492-501, (2014) (PubMed).

Target Details for MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Alternative Name: MYF5

Background:

Background Information: MYF5 is a member of the myogenic basic helix-loop-helix family of transcription factors, which can activate the muscle differentiation program.

Gene Name: Myogenic factor 5

Alternative Symbols: EORVA, bHLHc2

Protein Name: Myogenic factor 5

Molecular Weight: 28kDa

Gene ID: 4617

NCBI Accession: NP_005584

UniProt: P13349

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development