AHNAK antibody (N-Term)

Catalog No. ABIN263928

The Mouse Monoclonal anti-AHNAK antibody (Clone EM-09) (ABIN263928) specifically detects AHNAK in WB, IP, IF and IHC (fro). The antibody is reactive with Human and Mouse samples.

Quick Overview for AHNAK antibody (N-Term) (ABIN263928)

Target: AHNAK (AHNAK Nucleoprotein (AHNAK))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This AHNAK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: EM-09

Product Details anti-AHNAK Antibody

Binding Specificity: N-Term

Specificity: The antibody reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Characteristics: Synonyms: Desmoyokin, Neuroblast differentiation-associated protein AHNAK

Purification: Protein A Affinity Chromatography

Immunogen: Bacterially expressed fragment of N-terminal domain of human AHNAK1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. Western blot (Positive control: HeLa human cervix carcinoma cell line). Immunocytochemistry (Permeabilization is required, positive control: HeLa human cervixcarcinoma cell line). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 to -80 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for AHNAK

Target: AHNAK (AHNAK Nucleoprotein (AHNAK))

Background: AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear, it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.Synonyms: Desmoyokin, Neuroblast differentiation-associated protein AHNAK

Gene ID: 79026

UniProt: Q09666