AHNAK antibody (N-Term)

Catalog No. ABIN302097

Cited in 1 publication. The Mouse Monoclonal anti-AHNAK antibody (Clone EM-09) (ABIN302097) specifically detects AHNAK in WB, IP, ICC and IHC (fx). The antibody is reactive with Human and Mouse samples.

Quick Overview for AHNAK antibody (N-Term) (ABIN302097)

Target: AHNAK (AHNAK Nucleoprotein (AHNAK))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This AHNAK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Fixed) (IHC (fx))

Clone: EM-09

Product Details anti-AHNAK Antibody

Binding Specificity: N-Term

Purpose: Anti-AHNAK1 Purified

Cross-Reactivity (Details): Human, Mouse

Characteristics: The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Recommended dilution: 1 μg/mL, non-reducing conditions

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-AHNAK antibody (ABIN302097)

Johnson, Zhang, Adams, Phillips, Freitas, Froehner, Green-Church, Montanaro: "Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins." in: PLoS ONE, Vol. 7, Issue 8, pp. e43515, (2012) (PubMed).

Target Details for AHNAK

Target: AHNAK (AHNAK Nucleoprotein (AHNAK))

Alternative Name: AHNAK1

Background: AHNAK1 nucleoprotein,AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear, it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.,Desmoyokin

Gene ID: 79026

UniProt: Q09666