His Tag antibody

Catalog No. ABIN264410

The Mouse Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB.

Quick Overview for His Tag antibody (ABIN264410)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB)

Clone: IPA2C5-1

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Characteristics: Synonyms: 6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized withHis-His-His-His-His-His synthetic peptide and mouse myeloma cells.

Isotype: IgG2a

Application Details

Application Notes: Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHistidine Epitope Tag

Target Type: Tag

Background: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.