SNAP25 antibody

Catalog No. ABIN264461

The Mouse Monoclonal anti-SNAP25 antibody has been validated for WB and IHC (p). It is suitable to detect SNAP25 in samples from Human, Rat, Mouse, Chicken, Cow and Monkey.

Quick Overview for SNAP25 antibody (ABIN264461)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human, Rat, Mouse, Chicken, Cow, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: B-29-F9

Product Details anti-SNAP25 Antibody

Specificity: Does not stain reduced proteins.

Characteristics: Synonyms: SNAP-25, SUP, SNAP, Super Protein, Synaptosomal-associated protein 25,Synaptosomal-associated 25 kDa protein

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withpurified rat SNAP25 and mouse NS1 cells.

Isotype: IgG1

Application Details

Application Notes: Western blot (1-5 μg/mL). Immunohistochemistry on Paraformaldehyde fixed CNS tissues (1-5 μg/mL). Positive Control: Cerebellum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background: SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells. These two isoforms differ by 9 amino acids in the central portion of the protein.Synonyms: RIC4, SNAP, SNAP-25, SUP, Super Protein, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, ric-4

Gene ID: 6616

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport