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Details for Product No. ABIN264878

Acetylated Lysine antibody (Biotin)

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Antigen
Reactivity
»Alternatives Chemical
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Biotin
Application
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 6 references available
Catalog no. ABIN264878
Quantity 0.4 mL
Price
434.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Acetylated KLH
Isotype IgG
Specificity This antibody detects proteins containing Acetylated Lysine residues in SDS-PAGE immunoblots. No reaction to Non-Acetylated proteins.
Purification Affinity Chromatography
Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nepsilon-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).
Alternate names: AcK, acetyl Lysine, acetyl-Lysine
Application Notes Western blot (6). Immunoprecipitation (7). ELISA. Immunoflourescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS 0.09% Sodium Azide 50% Glycerol
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Hassig, Schreiber: "Nuclear histone acetylases and deacetylases and transcriptional regulation: HATs off to HDACs." in: Current opinion in chemical biology, Vol. 1, Issue 3, pp. 300-8, 1998 (PubMed).

Martínez-Balbás, Bauer, Nielsen et al.: "Regulation of E2F1 activity by acetylation." in: The EMBO journal, Vol. 19, Issue 4, pp. 662-71, 2000 (PubMed).

Chan, Krstic-Demonacos, Smith et al.: "Acetylation control of the retinoblastoma tumour-suppressor protein." in: Nature cell biology, Vol. 3, Issue 7, pp. 667-74, 2001 (PubMed).

Vigushin, Coombes: "Targeted histone deacetylase inhibition for cancer therapy." in: Current cancer drug targets, Vol. 4, Issue 2, pp. 205-18, 2004 (PubMed).

Yang: "Lysine acetylation and the bromodomain: a new partnership for signaling." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 10, pp. 1076-87, 2004 (PubMed).

Yang: "Multisite protein modification and intramolecular signaling." in: Oncogene, Vol. 24, Issue 10, pp. 1653-62, 2005 (PubMed).

Alternatives for antigen "Acetylated Lysine", type "Antibodies"
Hosts (18), (18)
Reactivities (21), (8), (7), (4), (3), (2), (1), (1), (1)
Applications (35), (31), (16), (15), (8), (5), (4), (1), (1), (1)
Conjugates (5), (4)
Epitopes (7), (3)
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