CHEK2 antibody

Catalog No. ABIN265349

The Rabbit Polyclonal anti-CHEK2 antibody is suitable to detect CHEK2 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for CHEK2 antibody (ABIN265349)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CHEK2 Antibody

Specificity: Chk2 (Q510) antibody detects endogenous levels of Chk2 protein. Species: Human. Others not tested.

Characteristics: Synonyms: CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2checkpoint homolog

Purification: Aff - Purified

Application Details

Application Notes: ELISA: 1: 20000~1: 40000. WB: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclindependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved inthese processes as regulators of Cdks. Chk1 and Chk2 both function as essentialcomponents in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response toDNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A,Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators bydephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown thatChk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylatedform of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation byChk1.

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle