Cullin 1 (CUL1) antibody
| Antigen |
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| Synonyms |
LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunoprecipitation (IP) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (6), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN265375 |
| Quantity |
100 µg (1,0 mg/ml) (Variants) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Cullin-1, CUL-1, CUL1
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Alternative name
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Cullin 1
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Gene ID
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8454
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Swiss-Prot
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Q13616
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Cross-Reactivity
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Human, Mouse (Murine)
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Description
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Cullin proteins comprise a distinct family of mediators that participate in the selectivetargeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is themammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitinligase complex designated SCF. The SCF(Skp1/CUL-1/F-box protein complex) consists ofSkp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determinesthe substrate specificity of the complex. CUL-1 mediated ubiquitination results in thedegradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associateswith the tumor suppressing protein VHL and elongin B to form VBC complexes, whichstructurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associatingwith Nedd-8, a ubiquitin-like protein, where it too functions as an active component of amultifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein(VACM-1), is also included in the cullin family as it shares substantial sequence homologywith CUL-1.
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Specificity
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Cullin 1 (Y761) antibody detects endogenous levels of Cullin 1 protein. Species: Human, Mouse. Others not tested.
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Application Notes
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ELISA: 1: 20000~1: 40000. WB: 1: 500~1: 1000. IHC: 1: 50~1: 200. IF: 1: 50~1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1,0 mg/ml
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Purification
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Aff - Purified
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
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Storage
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Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
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Research Area
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Apoptosis/Necrosis, Proteasome
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Restrictions
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For Research Use only
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Alternatives for antigen "Cullin 1 (CUL1)", type "Antibodies"
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Hosts
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Rabbit (33)
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Reactivities
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Human (30), Mouse (Murine) (24), Rat (Rattus) (18), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1), Pig (Porcine) (1)
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Applications
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Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunoprecipitation (IP) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (6), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2), C-Term AA 766-776 (1)
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