KRT16 antibody

Catalog No. ABIN265495

Product Details anti-KRT16 Antibody

Target: KRT16 (Keratin 16 (KRT16))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of Cytokeratin 16 protein. (region surroundign Ser451)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-KRT16 antibody (ABIN265495)

Yu, Ye, Zhong, Ding, Chen: "Effect of advanced oxidation protein products on articular cartilage and synovium in a rabbit osteoarthritis model." in: Orthopaedic surgery, Vol. 7, Issue 2, pp. 161-7, (2016) (PubMed).

Zhang, Han, Song, Luo, Jin, Mu, Su, Ji, Ren, Liu: "Interferon-? regulates the function of mesenchymal stem cells from oral lichen planus via indoleamine 2,3-dioxygenase activity." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 44, Issue 1, pp. 15-27, (2015) (PubMed).

Suga, Sugimura, Koshiishi, Yorifuji, Makino, Takeda: "Heparin/heparan sulfate/CD44-v3 enhances cell migration in term placenta-derived immortalized human trophoblast cells." in: Biology of reproduction, Vol. 86, Issue 5, pp. 134, 1-8, (2012) (PubMed).

Target Details for KRT16

Target: KRT16 (Keratin 16 (KRT16))

Alternative Name: Cytokeratin 16 (KRT16 Products)

Synonyms: CK16 antibody, FNEPPK antibody, K16 antibody, K1CP antibody, KRT16A antibody, NEPPK antibody, AI324768 antibody, Krt1-16 antibody, Ka16 antibody, KRT16 antibody, Krt16 antibody, keratin 16 antibody, keratin 16, type I S homeolog antibody, keratin, type I cytoskeletal 16 antibody, KRT16 antibody, Krt16 antibody, krt16.S antibody, LOC101108147 antibody, LOC100714013 antibody

Background: Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10 % of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating. Two cytokeratins, 6 and 16, are expressed when keratincytes are undergoing rapid turnover in various pathological states, wound healing, psoriasis and some carcinomasSynonyms: CK16, Cytokeratin-16, K16, KRT16, KRT16A, Keratin 16, Keratin type I cytoskeletal 16, Keratin-16

Molecular Weight: approx. 52 kDa

Gene ID: 3868

NCBI Accession: NP_005548

UniProt: P08779