MEF2A antibody

Catalog No. ABIN265517

The Rabbit Polyclonal anti-MEF2A antibody is suitable to detect MEF2A in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for MEF2A antibody (ABIN265517)

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MEF2A Antibody

Specificity: This antibody detects endogenous levels of MEF2A protein. (region surrounding Ala306)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MEF2A

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Alternative Name: MEF2A

Background: The myocyte enhancer factor-2 (MEF-2) family of transcription factors associated with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF4, are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.Synonyms: MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1

Molecular Weight: approx. 55 kDa

Gene ID: 4205

NCBI Accession: NP_001124398

UniProt: Q02078

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades