BAFF antibody

Catalog No. ABIN2688855

Product Details anti-BAFF Antibody

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAFF antibody is un-conjugated

Application: ELISA

Brand: BD Pharmingen™

Characteristics: Antibody H32-406 reacts with B-lymphocyte stimulator protein (Blys), also known as BAFF (B cell activating factor) and is a member of the TNF ligand superfamily. Members of the TNF receptor and ligand superfamily play a critical role in regulating immune responses. Blys is a type II membrane protein expressed on cells of myeloid lineage including monocytes, dendritic cells, and activated T cells. Blys exists in both a membrane-bound and soluble form. In its soluble form, Blys is a potent B cell growth factor in costimulation assays, suggesting its biological role in monocyte-driven B cell activation. Blys has also been linked to B cell hyperplasia and autoimmunity. Human Blys sandwich ELISA curve: The curve was generated by a sandwich ELISA using the purified H32-406 as capture antibody, doubling dilutions of recombinant human Blys protein and biotin H32-544 (Cat. No. 558290) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214)were used to develop the ELISA.

BD Pharmingen™ Purified Mouse anti-Human Blys (BAFF) - Purified - Clone H32-406 - Isotype Mouse IgG1, κ - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: H32

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-BAFF antibody (ABIN2688855)

Craxton, Magaletti, Ryan, Clark: "Macrophage- and dendritic cell--dependent regulation of human B-cell proliferation requires the TNF family ligand BAFF." in: Blood, Vol. 101, Issue 11, pp. 4464-71, (2003) (PubMed).

Mackay, Schneider, Rennert, Browning: "BAFF AND APRIL: a tutorial on B cell survival." in: Annual review of immunology, Vol. 21, pp. 231-64, (2003) (PubMed).

Khare, Sarosi, Xia, McCabe, Miner, Solovyev, Hawkins, Kelley, Chang, Van, Ross, Delaney, Wang, Lacey, Boyle, Hsu: "Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3370-5, (2000) (PubMed).

Moore, Belvedere, Orr, Pieri, LaFleur, Feng, Soppet, Charters, Gentz, Parmelee, Li, Galperina, Giri, Roschke, Nardelli, Carrell, Sosnovtseva, Greenfield, Ruben, Olsen, Fikes, Hilbert: "BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 260-3, (1999) (PubMed).

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: Blys BAFF (TNFSF13B Products)

Synonyms: BAFF antibody, BLYS antibody, CD257 antibody, DTL antibody, TALL-1 antibody, TALL1 antibody, THANK antibody, TNFSF20 antibody, ZTNF4 antibody, BAFF-R antibody, BAFFR antibody, BROMIX antibody, CD268 antibody, CVID4 antibody, prolixin antibody, TNFSF13B antibody, RGD1561519 antibody, RGD1560810 antibody, BLyS antibody, D8Ertd387e antibody, zTNF4 antibody, 2010006P15Rik antibody, Baffr antibody, Bcmd antibody, Bcmd-1 antibody, Bcmd1 antibody, Lvis22 antibody, zgc:172115 antibody, TNF superfamily member 13b antibody, TNF receptor superfamily member 13C antibody, tumor necrosis factor (ligand) superfamily, member 13b antibody, tumor necrosis factor receptor superfamily, member 13c antibody, tumor necrosis factor superfamily member 13b antibody, TNFSF13B antibody, TNFRSF13C antibody, Tnfsf13b antibody, Tnfrsf13c antibody, tnfsf13b antibody

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response