The M17/4 antibody reacts with the 180 kDa αL chain of LFA-1 (CD11a/CD18, αLβ2 integrin), a heterodimeric surface glycoprotein expressed on almost all leukocytes. CD8a+CD8b- intestinal intraepithelial T lymphocytes, which are believed to be thymus independent, do not express CD11a. LFA-1 mediates a variety of heterotypic and homotypic intercellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102), including participation in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. mAb M17/4 blocks a variety of LFA-1-mediated cells interactions in vitro, and costimulatory effects have also been described. In vivo treatment with M17/4 mAb reduces the severity of graft-versus-host reactions, prolongs allograft survival, inhibits the development of autoimmunity, and blocks substance P-induced leukocyte migration. The M17/4 and 2D7 (Cat. No. 553120) antibodies are reported to recognize different epitopes of the CD11a molecule. Immunohistochemical staining of CD11a positive cells. Frozen sections of mouse spleen were reacted with the anti-CD11a antibody. Leukocytes can be identified by the brown labeling of their cell membranes. Amplification 20X.
BD Pharmingen™ Purified Rat Anti-Mouse CD11a - Purified - Clone M17/4 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen
C57BL/6 Mouse Splenic Secondary Cytotoxic T Lymphocytes
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Zhao, Iwata: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." in: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).
Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).
Harning, Pelletier, Lubbe, Takei, Merluzzi: "Reduction in the severity of graft-versus-host disease and increased survival in allogenic mice by treatment with monoclonal antibodies to cell adhesion antigens LFA-1 alpha and MALA-2." in: Transplantation, Vol. 52, Issue 5, pp. 842-5, (1991) (PubMed).
Sanders, Vitetta: "B cell-associated LFA-1 and T cell-associated ICAM-1 transiently cluster in the area of contact between interacting cells." in: Cellular immunology, Vol. 132, Issue 1, pp. 45-55, (1991) (PubMed).
Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).
Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, (1983) (PubMed).
Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, (1983) (PubMed).