PD-1 antibody

Catalog No. ABIN2689141

Product Details anti-PD-1 Antibody

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The J43 antibody reacts with CD279 (PD-1), a 50-55- kDa glycoprotein encoded by the Pdcd1 gene of the CD28 family of the Ig superfamily. The expression of Pdcd1 mRNA and PD-1 protein is tightly regulated. PD-1 is transiently expressed on CD4-CD8thymocytes, it is upregulated on some cell lines upon induction of apoptosis, it is induced on thymocytes and splenic T and B lymphocytes after stimulation through their antigen receptors, and it is induced on activated myeloid cells. In addition, Pdcd1 mRNA is transiently expressed in developing B lymphocytes at the pro-B-cell stage. The presence of an ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) on PD-1's intracytoplasmic region and the development of splenomegaly and breakdown of peripheral tolerance in PD-1[-/-] mice suggest that PD-1 may be involved in the negative regulation of immune responses. The PD-1 ligands, B7-H1 (also known as PD-L1) and B7-DC (PD-L2), are members of the B7 family of the Ig superfamily. The J43 antibody blocks the binding of PD-1 to its two ligands.

BD Pharmingen™ Purified Hamster Anti-Mouse CD279 - Purified - Clone J43 - Isotype Armenian Hamster IgG2, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Syrian Hamster kidney cell line BKH transfected with Pdcd1 cDNA

Clone: J43

Isotype: IgG2 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-PD-1 antibody (ABIN2689141)

Tsushima, Iwai, Otsuki, Abe, Hirose, Yamazaki, Akiba, Yagita, Takahashi, Omura, Okumura, Azuma: "Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses." in: European journal of immunology, Vol. 33, Issue 10, pp. 2773-82, (2003) (PubMed).

Ansari, Salama, Chitnis, Smith, Yagita, Akiba, Yamazaki, Azuma, Iwai, Khoury, Auchincloss, Sayegh: "The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 63-9, (2003) (PubMed).

Carreno, Collins: "The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses." in: Annual review of immunology, Vol. 20, pp. 29-53, (2002) (PubMed).

Nishimura, Minato, Nakano, Honjo: "Immunological studies on PD-1 deficient mice: implication of PD-1 as a negative regulator for B cell responses." in: International immunology, Vol. 10, Issue 10, pp. 1563-72, (1999) (PubMed).

Nishimura, Agata, Kawasaki, Sato, Imamura, Minato, Yagita, Nakano, Honjo: "Developmentally regulated expression of the PD-1 protein on the surface of double-negative (CD4-CD8-) thymocytes." in: International immunology, Vol. 8, Issue 5, pp. 773-80, (1997) (PubMed).

Agata, Kawasaki, Nishimura, Ishida, Tsubata, Yagita, Honjo: "Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes." in: International immunology, Vol. 8, Issue 5, pp. 765-72, (1997) (PubMed).

Finger, Pu, Wasserman, Vibhakar, Louie, Hardy, Burrows, Billips: "The human PD-1 gene: complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors." in: Gene, Vol. 197, Issue 1-2, pp. 177-87, (1997) (PubMed).

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: CD279 (PDCD1 Products)

Synonyms: CD279 antibody, PD-1 antibody, PD1 antibody, SLEB2 antibody, hPD-1 antibody, hPD-l antibody, Ly101 antibody, Pdc1 antibody, programmed cell death 1 antibody, PDCD1 antibody, Pdcd1 antibody

Background: Synonyms: PD-1

Pathways: Cancer Immune Checkpoints