CD3 epsilon antibody

Catalog No. ABIN2689214

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Golden Syrian Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: This antibody has been reported to react with the 25- kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains. Plate-bound and soluble hamster anti-mouse CD3e (clone 500A2) antibody has been reported to activate unprimed T cells in vitro. Activation of a mouse T-cell clone by soluble hamster anti-mouse CD3e (clone 500A2) can be blocked by Fab fragments of anti-CD4 antibody GK1.5, suggesting that the hamster anti-mouse CD3e (clone 500A2) antibody may bind an epitope of CD3e close to a site at which CD4 associates with the T-cell receptor. This hamster mAb to a mouse leukocyte antigen has been reported not to cross-react with rat leukocytes. Immunohistochemical staining of T lymphocytes. A frozen section from mouse spleen was stained with the Hamster Anti-Mouse CD3e(Cat. No. 550277) antibody. T lymphocytes can be identified by the intense brown labeling of their cell surface membranes (20X magnification).

BD Pharmingen™ Purified Hamster Anti-Mouse CD3e - Purified - Clone 500A2 - Isotype Syrian Hamster IgG2, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse T-cell receptor

Clone: 500A2

Isotype: IgG2 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 62.5 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD3 epsilon antibody (ABIN2689214)

Havran, Poenie, Kimura, Tsien, Weiss, Allison: "Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes." in: Nature, Vol. 330, Issue 6144, pp. 170-3, (1987) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e (CD3E Products)

Synonyms: cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody

Background: Synonyms: CD3ε chain

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway