Selectin E/CD62e antibody (Biotin)

Catalog No. ABIN2689358

Product Details anti-Selectin E/CD62e Antibody (Biotin)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 10E9.6 antibody reacts with the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells. A suspension of TNFα stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen. The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNFα-treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD62E - Biotin - Clone 10E9.6 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse brain capillary endothelioma bEnd.3 (TNFalpha-stimulated) Cell Line

Clone: 10E9-6

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Selectin E/CD62e antibody (Biotin) (ABIN2689358)

Pendl, Robert, Steinert, Thanos, Eytner, Borges, Wild, Lowe, Fuhlbrigge, Kupper, Vestweber, Grabbe: "Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1." in: Blood, Vol. 99, Issue 3, pp. 946-56, (2002) (PubMed).

Borges, Pendl, Eytner, Steegmaier, Zöllner, Vestweber: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).

Ramos, Kunkel, Lawrence, Jung, Vestweber, Bosse, McIntyre, Gillooly, Norton, Wolitzky, Ley: "Differential effect of E-selectin antibodies on neutrophil rolling and recruitment to inflammatory sites." in: Blood, Vol. 89, Issue 8, pp. 3009-18, (1997) (PubMed).

Eppihimer, Wolitzky, Anderson, Labow, Granger: "Heterogeneity of expression of E- and P-selectins in vivo." in: Circulation research, Vol. 79, Issue 3, pp. 560-9, (1996) (PubMed).

Bosse, Vestweber: "Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum." in: European journal of immunology, Vol. 24, Issue 12, pp. 3019-24, (1995) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Weller, Isenmann, Vestweber: "Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha." in: The Journal of biological chemistry, Vol. 267, Issue 21, pp. 15176-83, (1992) (PubMed).

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: CD62E (SELE Products)

Synonyms: SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody

Background: Synonyms: E-selectin, ELAM-1

Pathways: Thromboxane A2 Receptor Signaling