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Antibody
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anti-RELA - NF-KB P65
RELA / NF-KB P65 antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN271750 |
| Quantity |
100 µg (1.0 mg/ml) (Variants) |
| Price |
320.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3, Nuclear factorNF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer inB-cells 3
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Swiss-Prot
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Q04206
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Description
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Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are membersof a family of transcription factors that include the two subunits of the transcription factorNFkappaB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteinsspecifically bind to DNA sequences that are the same or slight variations of the 10 bpkappaB sequence in the immunoglobulin kappa light chain enhancer. This same sequenceis also present in a number of other cellular and viral enhancers. The DNA binding activityof NFkappaB is activated and rapidly transported from the cytoplasm to the nucleus incells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct 50kDa proteins have been described, designated p105 and p100, respectively. The p105precursor contains p50 at its amino terminus and a C-terminal region that when expressedas a separate molecule, designated pdI, binds to p50 and regulates its activity.
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Specificity
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This antibody detects endogenous levels of NFkappaB-p65 protein. Species: Human and Mouse. Others not tested.
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Application Notes
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ELISA: 1/20000~1/40000. Western Blot: 1/500~1/1000. Immunohistochemistry: 1/50~1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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Phosphate buffered saline (PBS) approx. pH 7.2 containing 15 mM SodiumAzide as preservative.
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Storage
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Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "RELA / NF-KB P65", type "Antibodies"
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Hosts
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Rabbit (55), Mouse (5), Sheep (1)
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Reactivities
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Human (59), Mouse (Murine) (46), Rat (Rattus) (40), Rabbit (4), Monkey (3), Cow (Bovine) (2), Bird (Avian) (1), Dog (Canine) (1), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Enzyme Immunoassay (EIA) (18), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Electrophoretic Mobility-Shift Assay (EMSA) (4), Immunofluorescence (IF) (4), Flow Cytometry (FACS) (1)
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Epitopes
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N-Term (2), Center (1)
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