POU2F1 antibody

Catalog No. ABIN271753

Product Details anti-POU2F1 Antibody

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of OCT1/OTF1 protein. (region surrounding Leu68)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store Store the antibody undiluted at 2-8 °C.

Target Details for POU2F1

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Alternative Name: POU2F1 / OCT1 (POU2F1 Products)

Synonyms: OCT1 antibody, OTF1 antibody, oct-1B antibody, POU2F1 antibody, 2810482H01Rik antibody, NF-A1 antibody, Oct-1 antibody, Oct1 antibody, Otf-1 antibody, Otf1 antibody, chOCT-1 antibody, oct-1 antibody, oct1 antibody, pou2f1r antibody, fc07d06 antibody, wu:fc07d06 antibody, zgc:158360 antibody, POU class 2 homeobox 1 antibody, POU domain, class 2, transcription factor 1 antibody, POU class 2 homeobox 1 S homeolog antibody, POU class 2 homeobox 1b antibody, POU2F1 antibody, Pou2f1 antibody, pou2f1.S antibody, pou2f1b antibody

Background: Organic cation transporters (OCT) are expressed in the plasma membrane of epithelial cells from a wide range of tissues, where they function in the elimination of endogenous amines, cationic drugs and other xenobiotics. The structure of OCTs consists of a 12-transmembrane-domain structure and a large extracellular hydrophilic loop. In humans, OCT1 is primarily expressed in the liver while OCT2 is expressed in the kidney. OCT3 is expressed in the placenta, skeletal muscle, prostate, aorta and liver. OCT3, also known as extraneuronal monoamine transporter, is widely expressed in different regions of the brain including the hippocampus, cerebellum and cerebral cortex. OCT3 mediates the uptake of several neuroactive agents, including dopamine, and may play an important role in the disposition of neurotransmitters and cationic neurotoxins in the brain. The genes encoding human OCT1-3 map to a conserved cluster at chromosome 6q26-q27.Synonyms: NF-A1, OTF1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, Otf-1, POU domain class 2 transcription factor 1

Molecular Weight: approx. 89 kDa

Gene ID: 5451

NCBI Accession: NP_001185712

UniProt: P14859