FOXO1/3/4-pan antibody

Catalog No. ABIN271928

The Rabbit Polyclonal anti-FOXO1/3/4-pan antibody is suitable to detect FOXO1/3/4-pan in samples from Human, Mouse and Rat. It has been validated for IHC (p).

Quick Overview for FOXO1/3/4-pan antibody (ABIN271928)

Target: FOXO1/3/4-pan (FOXO1/3/4) (Forkhead Box O1/3/4 (FOXO1/3/4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1/3/4-pan antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO1/3/4-pan Antibody

Specificity: This antibody detects endogenous levels of FOXO1/3/4-pan protein. (region surrounding Pro18/26)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for FOXO1/3/4-pan

Target: FOXO1/3/4-pan (FOXO1/3/4) (Forkhead Box O1/3/4 (FOXO1/3/4))

Alternative Name: FOXO1/3/4-Pan

Background: FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined. FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia. FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.Synonyms: FOXO1A, FOXO3A, FOXO4A, Forkhead Box Protein O1A, Forkhead in Rhabdomyosarcoma

Molecular Weight: approx. 70 to 82 kDa