FOXO1/3/4-pan antibody (pThr24, pThr32)

Catalog No. ABIN6256521

Product Details anti-FOXO1/3/4-pan Antibody

Target: FOXO1/3/4-pan (FOXO1/3/4) (Forkhead Box O1/3/4 (FOXO1/3/4))

Binding Specificity: pThr24, pThr32

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1/3/4-pan antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Specificity: Phospho-FOXO1/3/4-pan (Thr24/32) Antibody detects endogenous levels of FOXO1/3/4-pan only when phosphorylated at Threonine 24/32

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human FOXO1/3/4-pan around the phosphorylation site of Threonine 24/32

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for FOXO1/3/4-pan

Target: FOXO1/3/4-pan (FOXO1/3/4) (Forkhead Box O1/3/4 (FOXO1/3/4))

Alternative Name: FOXO1/3/4-pan (FOXO1/3/4 Products)

Background:

Description: Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).

Gene: FOXO1

Molecular Weight: 78kDa

Gene ID: 2308

UniProt: Q12778, P98177