PIAS3 antibody

Catalog No. ABIN271953

The Rabbit Polyclonal anti-PIAS3 antibody (ABIN271953) specifically detects PIAS3 in WB and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PIAS3 antibody (ABIN271953)

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIAS3 Antibody

Specificity: This antibody detects endogenous levels of PIAS3 protein. (region surrounding Phe25)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for PIAS3

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Alternative Name: PIAS3

Background: The IL-6 type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization, and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.Synonyms: E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3

Molecular Weight: approx. 68 kDa

Gene ID: 10401

NCBI Accession: NP_006090

UniProt: Q9Y6X2

Pathways: JAK-STAT Signaling