DDIT3
Reactivity: Human
WB, IHC
Host: Rabbit
Monoclonal
unconjugated
Application Notes
ELISA: 1/1000-1/5000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.Synonyms: C/EBP-homologous protein, C/EBP-homologous protein 10, CHOP-10, CHOP10, DDIT-3, DDIT3, DNA damage-inducible transcript 3, Growth arrest and DNA-damage-inducible protein GADD153