ETS1 antibody

Catalog No. ABIN272023

Product Details anti-ETS1 Antibody

Target: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of ETS1 protein. (region surrounding Ala32)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for ETS1

Target: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Alternative Name: ETS1 (ETS1 Products)

Synonyms: ETS-1 antibody, EWSR2 antibody, ets1a-a antibody, XE1-a antibody, X1-c-ets-1a antibody, ETS-1A antibody, ETS-1B antibody, TNIP1 antibody, c-ets-1 antibody, c-ets1 antibody, Ets-1 antibody, Etsoncb antibody, Tpl1 antibody, ets1 antibody, ets antibody, AI196000 antibody, AI448617 antibody, D230050P06 antibody, cb516 antibody, id:ibd1116 antibody, zgc:110573 antibody, XE1-b antibody, ets-1 antibody, ets1-B antibody, ETS proto-oncogene 1, transcription factor antibody, v-ets avian erythroblastosis virus E26 oncogene homolog 1 S homeolog antibody, v-ets avian erythroblastosis virus E26 oncogene homolog 1 antibody, v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) antibody, E26 avian leukemia oncogene 1, 5' domain antibody, v-ets avian erythroblastosis virus E26 oncogene homolog 1 L homeolog antibody, ETS1 antibody, ets1.S antibody, Ets1 antibody, ets1 antibody, ets1.L antibody

Background: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation.Synonyms: EWSR2, Protein C-ets-1, p54

Molecular Weight: approx. 54 kDa

Gene ID: 2113

NCBI Accession: NP_005229

UniProt: P14921