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Antibody
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anti-RELA - NF-KB P65
RELA / NF-KB P65 antibody
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
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| Catalog no. |
ABIN272045 |
| Quantity |
100 µg (1.0 mg/ml) (Variants) |
| Price |
320.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3, Nuclear factorNF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer inB-cells 3
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Swiss-Prot
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Q04206
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Description
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P65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widelyrecognized as a critical mediator of immune and inflammatory responses. In most celltypes, NFkB is found in the cytoplasm where it is associated with an inhibitory proteinknown as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cellactivation signals, bacterial endotoxins, viral transforming proteins, certain growth factorsand reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by theinduced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkBregulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteinsinvolved in coagulation and genes involved in cell growth control. Additionally, NFkB isthought to be an important transcriptional regulator for HIV. Growing evidence indicatesthat the dysregulation of NFkB may be key to a number of diseases including arthritis andother inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.
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Specificity
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NFkappaB-p65 (A499) antibody detects endogenous levels of NFkappaB-p65 protein. Species: Human, Mouse, Rat. Others not tested.
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Application Notes
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ELISA: 1: 40000~1: 60000. Western blot: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2
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Storage
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Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "RELA / NF-KB P65", type "Antibodies"
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Hosts
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Rabbit (55), Mouse (5), Sheep (1)
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Reactivities
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Human (59), Mouse (Murine) (46), Rat (Rattus) (39), Rabbit (4), Monkey (3), Cow (Bovine) (2), Bird (Avian) (1), Dog (Canine) (1), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), Enzyme Immunoassay (EIA) (18), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Electrophoretic Mobility-Shift Assay (EMSA) (4), Immunofluorescence (IF) (4), Flow Cytometry (FACS) (1)
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Epitopes
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N-Term (2), Center (1)
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