GTF3C2 antibody

Catalog No. ABIN272244

The Rabbit Polyclonal anti-GTF3C2 antibody (ABIN272244) specifically detects GTF3C2 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for GTF3C2 antibody (ABIN272244)

Target: GTF3C2 (General Transcription Factor IIIC, Polypeptide 2, beta 110kDa (GTF3C2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF3C2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GTF3C2 Antibody

Specificity: This antibody detects endogenous levels of TFIIIC110 protein. (region surrounding Arg95)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for GTF3C2

Target: GTF3C2 (General Transcription Factor IIIC, Polypeptide 2, beta 110kDa (GTF3C2))

Alternative Name: GTF3C2 / TFIIIC110

Background: RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1) and TFIIIB", the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: General transcription factor 3C polypeptide 2, KIAA0011, TF3C-beta, Transcription factor IIIC 110 kDa subunit, Transcription factor IIIC subunit beta

Molecular Weight: approx. 100 kDa

Gene ID: 2976

NCBI Accession: NP_001030598

UniProt: Q8WUA4