LIG4 antibody

Catalog No. ABIN272301

The Rabbit Polyclonal anti-LIG4 antibody (ABIN272301) specifically detects LIG4 in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for LIG4 antibody (ABIN272301)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LIG4 Antibody

Specificity: This antibody detects endogenous levels of DNA Ligase IV protein. (region surrounding Gly619)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: DNA Ligase 4

Background: The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These proteins form a critical complex consisting of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligate accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4/ DNA Ligase IV complex is also involved in V(D)J recombination. V(D)J recombination occurs in normal development of the adaptive immune system and involves the formation of a double-strand break intermediate. Deletions of either DNA Ligase IV or XRCC4 inhibit the completion of V(D)J recombination, resulting in a high incidence of apoptosis in the developing nervous system and a block in B and T cell maturation.Synonyms: DNA ligase IV, EC=6.5.1.1, LIG4, Polydeoxyribonucleotide synthase [ATP] 4

Molecular Weight: 104 kDa

Gene ID: 3981

NCBI Accession: NP_001091738

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response