TNNI3 antibody

Catalog No. ABIN272322

Product Details anti-TNNI3 Antibody

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Human, Mouse, Cow, Rabbit, Fish, Chicken, Amphibian

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts equally with free cardiac Troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 186 and 192 of cTnI. Cross-reacts with skeletal troponin I from human, rat and mouse.

Cross-Reactivity (Details): Also reacts to: skeletal

Purification: Protein A chromatography

Purity: > 90 % pure (SDS-PAGE)

Immunogen: Free human cardiac troponin I

Clone: C5

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 3.5mg/mL (Sigma protein assay kit)

Buffer: PBS, pH 7.4 containing 0,09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-TNNI3 antibody (ABIN272322)

Laube, Heister, Scholz, Borchardt, Braun: "Re-programming of newt cardiomyocytes is induced by tissue regeneration." in: Journal of cell science, Vol. 119, Issue Pt 22, pp. 4719-29, (2007) (PubMed).

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: Cardiac Troponin I (TNNI3 Products)

Synonyms: CMD1FF antibody, CMD2A antibody, CMH7 antibody, RCM1 antibody, TNNC1 antibody, cTnI antibody, CMD1Z antibody, CMH13 antibody, TN-C antibody, TNC antibody, TNNC antibody, Tn1 antibody, TnIc antibody, ctnIc antibody, XTnIc antibody, c-troponin antibody, cTNT antibody, cmh7 antibody, ctni antibody, tnnc1 antibody, TNNI3 antibody, TnI antibody, cTNI antibody, AI874626 antibody, TnC antibody, cTnC antibody, tncc antibody, Tncc antibody, troponin I3, cardiac type antibody, troponin C1, slow skeletal and cardiac type antibody, troponin I, cardiac 3 antibody, troponin I type 3 (cardiac) antibody, troponin I3, cardiac type S homeolog antibody, troponin I3, cardiac type L homeolog antibody, cardiac troponin I antibody, troponin C, cardiac/slow skeletal antibody, TNNI3 antibody, TNNC1 antibody, Tnni3 antibody, tnni3.S antibody, tnni3.L antibody, tnni3 antibody, LOC100462680 antibody, Tnnc1 antibody

Background: Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Synonyms: TNNC1, TNNI3

Gene ID: 7137

NCBI Accession: NP_000354

UniProt: P19429