HIS Tag antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (109), ELISA (74), Immunoprecipitation (IP) (48), Immunofluorescence (IF) (45), Flow Cytometry (FACS) (31), Immunocytochemistry (ICC) (12), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), Transfected cell culture (TCC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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| Catalog no. |
ABIN274242 |
| Quantity |
50ug (100µg/100µl) (Variants) |
| Price |
226.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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His-Tag
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Immunogen
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The antiserum was produced by immunizing rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H).
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Specificity
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His-Tag polyclonal antibody detects His-tagged proteins overexpressed in cells. The antibody recognizes the His-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.
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Application Notes
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WB: 1:500~1:1000
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Concentration
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100µg/100µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
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Buffer
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Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20° C /1 year
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Research Area
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Tags/Labels
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Restrictions
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For Research Use only
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Alternatives for antigen "HIS Tag", type "Antibodies"
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Hosts
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Mouse (78), Rabbit (53), Chicken (11), Goat (7), Sheep (1)
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Reactivities
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Fusion Protein (Tag) (14), Human (11), All Species (9), Mouse (Murine) (8), Rat (Rattus) (6), Tag (4), Cow (Bovine) (1), Dog (Canine) (1), Pig (Porcine) (1), Rabbit (1)
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Applications
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Western Blotting (WB) (109), ELISA (74), Immunoprecipitation (IP) (48), Immunofluorescence (IF) (45), Flow Cytometry (FACS) (31), Immunocytochemistry (ICC) (12), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), Transfected cell culture (TCC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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Conjugates
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HRP (12), Biotin (10), FITC (8), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alkaline Phosphatase (AP) (2), HiLyte Fluor 488 (2), HiLyte Fluor 555 (2), HiLyte Fluor 647 (2), PE,Cy5.5 (2), AMCA (1), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FAM (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), ROX (1), Sepharose (1), TAMRA (1)
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Alternatives 
