The mouse monoclonal antibody H398 recognizes the extracellular domain of CD120a, a 55 kDa receptor for tumor necrosis factor. The antibody blocks biological activity of both natural and recombinant human TNF alpha and TNF beta.
Cross-Reactivity (Details)
Human
Purification
Purified by protein-A affinity chromatography.
Purity
> 95 % (by SDS-PAGE)
Endotoxin Level
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Baker, Pettitt, Slupsky, Chen, Glenn, Zuzel, Cawley: "Response of hairy cells to IFN-alpha involves induction of apoptosis through autocrine TNF-alpha and protection by adhesion." in: Blood, Vol. 100, Issue 2, pp. 647-53, (2002) (PubMed).
Kohrgruber, Halanek, Gröger, Winter, Rappersberger, Schmitt-Egenolf, Stingl, Maurer: "Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3250-9, (1999) (PubMed).
Target
TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
TNF receptor superfamily member 1A,CD120a / TNF R1, also known as TNFR55 or TNFRSF1A, is a 55 kDa receptor for tumor necrosis factor alpha and it is expressed in most tissues. By binding its trimeric ligand the CD120a protein forms trimers and the conformation change leads to dissociation of the inhibitory factor SODD from its intracellular death domain and in formation of signaling platform. CD120a can mediate apoptosis, and function as a regulator of inflammation. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.,TNF R1, TNFRSF1A, TNFR55, FPF, TBP1, p60