KLHL12 antibody (N-Term)

Catalog No. ABIN2775905

Product Details anti-KLHL12 Antibody

Target: KLHL12 (Kelch-Like 12 (Drosophila) (KLHL12))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLHL12 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EKGKPYVDIQ GLTASTMEIL LDFVYTETVH VTVENVQELL PAACLLQLKG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against KLHL12. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human KLHL12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for KLHL12

Target: KLHL12 (Kelch-Like 12 (Drosophila) (KLHL12))

Alternative Name: KLHL12 (KLHL12 Products)

Synonyms: zgc:92570 antibody, wu:fi60g03 antibody, C3ip1 antibody, C3IP1 antibody, DKIR antibody, hDKIR antibody, Ski2 like RNA helicase antibody, kelch like family member 12 L homeolog antibody, kelch like family member 12 antibody, kelch-like family member 12 antibody, kelch-like 12 antibody, SKIV2L antibody, klhl12.L antibody, KLHL12 antibody, klhl12 antibody, Klhl12 antibody

Background: KLHL12 is a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4.
Alias Symbols: C3IP1, DKIR, hDKIR
Protein Interaction Partner: PEF1, TBC1D10C, FGD2, SYNE4, CCBE1, TTBK2, RPP25L, GLYCTK, MRPL10, SNX20, C1QTNF2, ZBP1, KLHL12, WDYHV1, PRR13, CARD10, KLHL3, INMT, CUL3, BAG6, VEGFB, UBE2I, SDCBP, PDGFRB, MPP1, FLNA, TAOK2, DGCR6, DROSHA, EZH2, KLHL2, DVL3, UBC, ATXN1, CAND1, DCUN1D1,
Protein Size: 568

Molecular Weight: 63 kDa

Gene ID: 59349

NCBI Accession: NM_021633, NP_067646

UniProt: Q53G59