FZD8 antibody (Frizzled Family Receptor 8) (N-Term)

Details for Product anti-FZD8 Antibody No. ABIN2776728, Supplier: Log in to see
Antigen
  • FZ-8
  • FZD8
  • cFz-8
  • Fz-8
  • frizzled8
  • xfz8
  • Xfz8
  • frizzled-8
  • hfz8
  • hFZ8
  • fzd8
  • Fz8
  • frizzled family receptor 5
  • frizzled family receptor 8
  • frizzled homolog 8 (Drosophila)
  • FZD5
  • fzd8-b
  • FZD8
  • fzd8
  • Fzd8
  • fzd8-a
Alternatives
anti-Human FZD8 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
N-Term
19
14
6
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
64
49
23
7
6
5
3
3
2
2
Host
Rabbit
47
21
17
1
Clonality
Polyclonal
Conjugate
This FZD8 antibody is un-conjugated
4
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
61
33
26
23
8
4
1
1
Options
Supplier
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Supplier Product No.
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human FZD8
Blocking Peptide Blocking peptide for this product available: ABIN977174
Sequence PDTLCMDYNR TDLTTAAPSP PRRLPPPPPG EQPPSGSGHG RPPGARPPHR
Predicted Reactivity Dog: 100%, Human: 100%, Mouse: 93%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against FZD8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name FZD8 (FZD8 Antibody Abstract)
Background FZD8 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.
Alias Symbols: FZ-8, hFZ8
Protein Interaction Partner: WNT1, LRP5, LRP6, SUMO1, GOPC, RYK,
Protein Size: 694
Molecular Weight 73 kDa
Gene ID 8325
NCBI Accession NM_031866, NP_114072
UniProt Q9H461
Pathways WNT Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 694 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Frizzled Family Receptor 8 (FZD8) (N-Term) antibody (ABIN2776728) WB Suggested Anti-FZD8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Product cited in: Xu, Wen, Liu, Xu, Yang, Liu, Feng, An: "MicroRNA-375 suppresses human colorectal cancer metastasis by targeting Frizzled 8." in: Oncotarget, Vol. 7, Issue 26, pp. 40644-40656, 2016 (PubMed).

Yin, Xu, Bonfil, Banerjee, Sarkar, Sethi, Reddy: "Tumor-initiating cells and FZD8 play a major role in drug resistance in triple-negative breast cancer." in: Molecular cancer therapeutics, Vol. 12, Issue 4, pp. 491-8, 2013 (PubMed).

Background publications Lu, Yamamoto, Ortega, Baltimore: "Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth." in: Cell, Vol. 119, Issue 1, pp. 97-108, 2004 (PubMed).

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