SETDB1 antibody (N-Term)

Catalog No. ABIN2780883

Product Details anti-SETDB1 Antibody

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Binding Specificity: N-Term

Reactivity: Human, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PGCIGLDAAT ATVESEEIAE LQQAVVEELG ISMEELRHFI DEELEKMDCV

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Rabbit: 85%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against SETDB1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETDB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Alternative Name: SETDB1 (SETDB1 Products)

Synonyms: ESET antibody, H3-K9-HMTase4 antibody, KG1T antibody, KMT1E antibody, TDRD21 antibody, AU022152 antibody, mKIAA0067 antibody, SETDB1 antibody, NV10069 antibody, fc07d03 antibody, si:ch211-81a5.6 antibody, wu:fc07d03 antibody, im:7143091 antibody, wu:fa04a12 antibody, wu:fj80h02 antibody, zgc:152899 antibody, SET domain bifurcated 1 antibody, SET domain, bifurcated 1 antibody, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11 antibody, histone-lysine N-methyltransferase SETDB1 antibody, SET domain, bifurcated 1a antibody, SET domain bifurcated 1 L homeolog antibody, SET domain, bifurcated 1b antibody, SETDB1 antibody, Setdb1 antibody, MLLT11 antibody, LOC100120014 antibody, LOC100461671 antibody, setdb1a antibody, setdb1.L antibody, setdb1b antibody

Background: SETDB1 is a histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Protein Interaction Partner: MDM2, HSP90AA1, UBC, SKIL, LRIF1, BARD1, APLP1, TCERG1, TRIM28, VIM, SUMO2, PML, CLSTN1, JARID2, tat, MZT2B, FAM118B, MRPL44, LUC7L2, MAP4K5, TSC22D1, TTR, TK1, SMN1, PCYT2, PSME1, GRB7, CDKN1A, CDK4, BID, ANXA7, ZNF274, HIST1H3A, ESCO1, AKT2, AKT1, PPARG
Protein Size: 397

Molecular Weight: 43 kDa

Gene ID: 9869