POMT1 antibody (Middle Region)

Catalog No. ABIN2782091

Product Details anti-POMT1 Antibody

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Pig, Rat, Cow, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LTFQILLLPV VLQHISDHLC RSQLQRSIFS ALVVAWYSSA CHVSNTLRPL

Predicted Reactivity: Cow: 86%, Dog: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 86%, Rabbit: 77%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against POMT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POMT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 725 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POMT1

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Alternative Name: POMT1 (POMT1 Products)

Synonyms: LGMD2K antibody, MDDGA1 antibody, MDDGB1 antibody, MDDGC1 antibody, RT antibody, AI505244 antibody, protein O-mannosyltransferase 1 antibody, protein-O-mannosyltransferase 1 antibody, POMT1 antibody, Pomt1 antibody

Background: POMT1 is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K).O-mannosylation is an important protein modification in eukaryotes that is initiated by an evolutionarily conserved family of protein O-mannosyltransferases. POMT1 shares sequence similarity with protein O-mannosyltransferases of S. cerevisiae. In yeast, these enzymes are located in the endoplasmic reticulum (ER) and are required for cell integrity and cell wall rigidity. POMT1 also shows similarity to the Drosophila 'rotated abdomen' (rt) gene, which when mutated causes defects in myogenesis and muscle structure.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-42 DB154785.1 1-42 43-2420 AK074874.1 1-2378 2421-2617 BF984005.1 438-634 2618-3080 AL358781.19 127610-128072
Alias Symbols: FLJ37239, LGMD2K, RT, MDDGA1, MDDGB1, MDDGC1
Protein Interaction Partner: UBC, POMT2,
Protein Size: 725

Molecular Weight: 82 kDa

Gene ID: 10585

NCBI Accession: NM_001077365, NP_001070833

UniProt: B3KQG0