GAD antibody (N-Term)

Catalog No. ABIN2785666

Product Details anti-GAD Antibody

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MASSTPSSSA TSSNAGADPN TTNLRPTTYD TWCGVAHGCT RKLGLKICGF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GAD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GAD1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 594 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1 (GAD1 Products)

Synonyms: CPSQ1 antibody, GAD antibody, SCP antibody, GAD-67 antibody, cpsq1 antibody, gad1 antibody, gad1-A antibody, gad67 antibody, GAD67 antibody, GAD1 antibody, GLUTAMATE DECARBOXYLASE antibody, GLUTAMATE DECARBOXYLASE 1 antibody, MKP11.30 antibody, MKP11_30 antibody, glutamate decarboxylase antibody, EP10 antibody, GAD25 antibody, GAD44 antibody, Gad-1 antibody, Gad67 antibody, glutamate decarboxylase 1 antibody, glutamate decarboxylase 1 L homeolog antibody, glutamate decarboxylase antibody, glutamate decarboxylase 1b antibody, GAD1 antibody, gad1.1.L antibody, GAD antibody, Gad1 antibody, gad1b antibody

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Alias Symbols: FLJ45882, GAD, SCP, CPSQ1
Protein Interaction Partner: CMTM5, AGTRAP, HAAO, STK3, GAD2, PPP2CA, PRKCE, PPP2CB, PRKACA,
Protein Size: 594

Molecular Weight: 67 kDa

Gene ID: 2571

NCBI Accession: NM_000817, NP_000808

UniProt: Q99259