MIS12 antibody (Middle Region)

Catalog No. ABIN2787627

Product Details anti-MIS12 Antibody

Target: MIS12 (MIS12, MIND Kinetochore Complex Component, Homolog (MIS12))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIS12 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TLTFFDELHN VGRDHGTSDF RESLVSLVQN SRKLQNIRDN VEKESKRLKI

Predicted Reactivity: Cow: 86%, Horse: 86%, Human: 100%, Mouse: 91%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MIS12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MIS12

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 205 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MIS12

Target: MIS12 (MIS12, MIND Kinetochore Complex Component, Homolog (MIS12))

Alternative Name: MIS12 (MIS12 Products)

Synonyms: 2510025F08Rik antibody, KNTC2AP antibody, MTW1 antibody, hMis12 antibody, BcDNA:RE19545 antibody, CG18156 antibody, Dmel\\CG18156 antibody, Mis-12 antibody, dmMis12 antibody, AU044651 antibody, Misc12 antibody, RP23-83I13.9 antibody, mtw1 antibody, kntc2ap antibody, MGC80789 antibody, ARABIDOPSIS MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE antibody, ATMIS12 antibody, MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE antibody, MINICHROMOSOME INSTABILITY 12 HOMOLOGUE antibody, MIS12 HOMOLOGUE antibody, MOK9.12 antibody, MOK9_12 antibody, MIS12, kinetochore complex component antibody, CG18156 gene product from transcript CG18156-RA antibody, MIS12 kinetochore complex component antibody, MIS12 kinetochore complex component L homeolog antibody, MIS12, MIND kinetochore complex component, homolog (S. pombe) antibody, minichromosome instability 12 (mis12)-like protein antibody, kinetochore protein Mis12 antibody, MIS12 antibody, Mis12 antibody, mis12.L antibody, mis12 antibody

Background: MIS12 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis.
Alias Symbols: 2510025F08Rik, KNTC2AP, MGC2488, MTW1, hMis12
Protein Interaction Partner: PMF1, ZWINT, SUGT1, CBX5, CASC5, NSL1, NDC80, NF1, BUB1, DSN1, BUB1B, HSP90AA1, SKA1, UBC, SPC24, NUF2, MIS12, SPC25, TF, PIGR, SERPINA1, HP, FGB, FGA, CENPC, APOA1, CBX3,
Protein Size: 205

Molecular Weight: 23 kDa

Gene ID: 79003

NCBI Accession: NM_024039, NP_076944

UniProt: Q9H081

Pathways: M Phase