GTF2F1 antibody (N-Term)

Catalog No. ABIN2792594

Product Details anti-GTF2F1 Antibody

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2F1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against GTF2F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GTF2F1

Application Details

Application Notes: WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 µg/mL
Positive Control: K562 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 517 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2F1

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Alternative Name: GTF2F1 / RAP74 (GTF2F1 Products)

Synonyms: BTF4 antibody, RAP74 antibody, TF2F1 antibody, TFIIF antibody, C76800 antibody, fd13c08 antibody, rap74 antibody, wu:fd13c08 antibody, zgc:56155 antibody, AO090124000085 antibody, DDBDRAFT_0205751 antibody, DDBDRAFT_0302526 antibody, DDB_0205751 antibody, DDB_0302526 antibody, 2810405L04Rik antibody, ATRAP74 antibody, T1P17.200 antibody, T1P17_200 antibody, general transcription factor IIF subunit 1 antibody, general transcription factor IIF, polypeptide 1 antibody, transcription initiation factor IIF subunit alpha antibody, general transcription factor IIF subunit 1 L homeolog antibody, transcription initiation factor IIF subunit alpha RAP74 antibody, GTF2F1 antibody, gtf2f1 antibody, AOR_1_160104 antibody, Gtf2f1 antibody, gtf2f1.L antibody, RAP74 antibody

Background: GTF2F1 is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
Alias Symbols: BTF4, RAP74, TF2F1, TFIIF
Protein Interaction Partner: ABL1, UBC, tat, PUS1, DUS3L, VPS29, SNF8, PSAP, BCAT1, TCEA1, GTF2B, POLR2C, GTF2F2, CDC73, MMS19, SF3A3, SRSF9, COIL, SNRPA, POLR2A, EEF1B2, CIRBP, CDK9, POLR2D, GTF2H1, GTF2E1, ERCC2, CDK8, CCNC, MAGEC1, TAF1, HNRNPU, FCP1, CSNK2A1, CCNT1, Gtf3c6, POLR2
Protein Size: 517

Molecular Weight: 58 kDa

Gene ID: 2962

NCBI Accession: NM_002096, NP_002087

UniProt: P35269