TPX2 antibody (AA 350-382)

Catalog No. ABIN2796197

Product Details anti-TPX2 Antibody

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Binding Specificity: AA 350-382

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit Anti-Human P100 Antibody

Immunogen: This P100 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-382 amino acids from human P100.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100The anti-p100 Pab is used in Western blot to detect p100 in placenta lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for TPX2

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Alternative Name: P100 (TPX2 Products)

Synonyms: C20orf1 antibody, C20orf2 antibody, DIL-2 antibody, DIL2 antibody, FLS353 antibody, GD:C20orf1 antibody, HCA519 antibody, HCTP4 antibody, REPP86 antibody, p100 antibody, 2610005B21Rik antibody, TPX2 antibody, dil-2 antibody, dil2 antibody, hctp4 antibody, repp86 antibody, tpx2 antibody, TPX2, microtubule nucleation factor antibody, TPX2, microtubule-associated antibody, TPX2, microtubule-associated, homolog (Xenopus laevis) antibody, TPX2, microtubule-associated S homeolog antibody, TPX2, microtubule-associated L homeolog antibody, TPX2 antibody, Tpx2 antibody, tpx2.S antibody, tpx2.L antibody

Background:

Target Description: The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. The full-length cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif. RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney. P100 is also correlated to cancer prognosis.

Gene Symbol: TPX2

Molecular Weight: 85653 Da

Gene ID: 22974

UniProt: Q9ULW0