SMAD2 antibody

Catalog No. ABIN1944918

This anti-SMAD2 antibody is a Rabbit Polyclonal antibody detecting SMAD2 in WB. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for SMAD2 antibody (ABIN1944918)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD2 Antibody

Isotype: IgG

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-SMAD2 antibody (ABIN1944918)

Liu, Pouponnot, Massagué: "Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes." in: Genes & development, Vol. 11, Issue 23, pp. 3157-67, (1998) (PubMed).

Takenoshita, Mogi, Nagashima, Yang, Yagi, Hanyu, Nagamachi, Miyazono, Hagiwara: "Characterization of the MADH2/Smad2 gene, a human Mad homolog responsible for the transforming growth factor-beta and activin signal transduction pathway." in: Genomics, Vol. 48, Issue 1, pp. 1-11, (1998) (PubMed).

Zhang, Feng, We, Derynck: "Receptor-associated Mad homologues synergize as effectors of the TGF-beta response." in: Nature, Vol. 383, Issue 6596, pp. 168-72, (1996) (PubMed).

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Smad2

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Molecular Weight: 52306 Da

Gene ID: 4087

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus