NPT II antibody

Catalog No. ABIN285052

Product Details

Target: NPT II

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: MPTII antibody was raised in rabbit using purified neomycin phosphotransferase II as the immunogen.

Isotype: IgG

Application Details

Application Notes: Use at starting dilution 1:500
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Supplied as an IgG fraction in PBS, pH 7.4 with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -80 °C for long term storage.

References

Fu, Guo, Alfano: "Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin." in: Journal of bacteriology, Vol. 188, Issue 17, pp. 6060-9, (2006) (PubMed).

Target Details

Target: NPT II

Alternative Name: NPTII

Background: Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.