Perforin 1 antibody

Catalog No. ABIN285909

The Rabbit Polyclonal anti-Perforin 1 antibody (ABIN285909) specifically detects Perforin 1 in WB. The antibody is reactive with Rat samples.

Quick Overview for Perforin 1 antibody (ABIN285909)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Perforin 1 Antibody

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Reacts with both rat and mouse perforin. Cross-reactivity to perforin of other species has not been tested.

Purification: purified

Immunogen: Perforin antibody was raised in rabbit using E. coli-expressed rat perforin as the immunogen.

Application Details

Application Notes: WB: 1:1,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitue in distilled water.

Concentration: Lot specific

Buffer: Supplied as a lyophilized Potein A purified rabbit IgG with 0.1 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Background: Perforin-1 is a protein that in humans is encoded by the PRF1 gene. Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria.

Pathways: Apoptosis, Caspase Cascade in Apoptosis