Perforin 1 antibody (FITC)

Catalog No. ABIN7506004

The Mouse Monoclonal anti-Perforin 1 antibody is suitable to detect Perforin 1 in samples from Human and Cow. It has been validated for ICFC.

Quick Overview for Perforin 1 antibody (FITC) (ABIN7506004)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is conjugated to FITC

Application: Intracellular Flow Cytometry (ICFC)

Clone: DG9

Product Details anti-Perforin 1 Antibody (FITC)

Purpose: Anti-Perforin FITC

No Cross-Reactivity: Mouse

Characteristics: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: purified granules from human YT lymphoma cell line

Isotype: IgG2b, kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Background: Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2

Gene ID: 5551

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis