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Perforin 1 antibody

PRF1 Reactivity: Human, Cow FACS, IP, ICC, IHC (fro), IHC (p) Host: Mouse Monoclonal dG9 unconjugated
Catalog No. ABIN1981903
  • Target See all Perforin 1 (PRF1) Antibodies
    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
    Reactivity
    • 79
    • 26
    • 9
    • 7
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    Human, Cow
    Host
    • 57
    • 40
    • 4
    Mouse
    Clonality
    • 58
    • 43
    Monoclonal
    Conjugate
    • 57
    • 13
    • 11
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Perforin 1 antibody is un-conjugated
    Application
    • 50
    • 40
    • 30
    • 19
    • 14
    • 9
    • 7
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
    No Cross-Reactivity
    Mouse
    Cross-Reactivity (Details)
    Human, Bovine
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    purified granules from human YT lymphoma cell line
    Clone
    DG9
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product PRF1 Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 3-12 μg/mL. Intracellular staining.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze. Do not use after expiration date stamped on vial label.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Li, Jeong, Song: "Molecular Characteristics and Distribution of Adult Human Corneal Immune Cell Types." in: , Vol. 13, pp. 798346, (2022) (PubMed).

    Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).

    Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).

    Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).

  • Target
    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
    Alternative Name
    Perforin (PRF1 Products)
    Synonyms
    FLH2 antibody, HPLH2 antibody, P1 antibody, PFN1 antibody, PFP antibody, PRF1 antibody, Pfn antibody, Pfp antibody, Prf-1 antibody, Cyta antibody, RATCYTA antibody, LOC443187 antibody, perforin antibody, prf1 antibody, cytolysin antibody, perforin-1 antibody, perforin-1-like antibody, perforin 1 antibody, perforin 1 (pore forming protein) antibody, perforin antibody, perforin 1 L homeolog antibody, PRF1 antibody, Prf1 antibody, LOC443187 antibody, prf1 antibody, prf1.L antibody
    Background
    Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2
    Gene ID
    5551
    UniProt
    P14222
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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