COL6 antibody

Catalog No. ABIN289406

The Rabbit Polyclonal anti-COL6 antibody is suitable to detect COL6 in samples from Human. It has been validated for WB, IHC, ELISA and IP.

Quick Overview for COL6 antibody (ABIN289406)

Target: COL6 (Collagen, Type VI (COL6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-COL6 Antibody

Cross-Reactivity (Details): Less than 1% crossreactivity with Type I, II, III, IV and V collagens.

Characteristics: Affinity purified Rabbit polyclonal Collagen Type VI antibody
Bioactivity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues

Purification: Affinity chromatography purified

Sterility: Filtered

Immunogen: Collagen type VI antibody was raised in rabbit using collagen type VI from human and bovine placenta as the immunogen.

Application Details

Application Notes: ELISA: 1:4,000-1:8,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid in 0.125 M Na5 5 7, pH 8.0, with 0.075 NaCl, 0.005 EDTA, and 0.01 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for COL6

Target: COL6 (Collagen, Type VI (COL6))

Background: Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Collagen alpha-1(VI) chain is a protein that in humans is encoded by the COL6A1 gene. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively.