UCP1 antibody

Catalog No. ABIN289562

The Rabbit Polyclonal anti-UCP1 antibody is suitable to detect UCP1 in samples from Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for UCP1 antibody (ABIN289562)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-UCP1 Antibody

Specificity: This antibody is 100% conserved in Human, Rat and Mice and 94 % in Bovine

Cross-Reactivity (Details): This UCP1 peptide sequence is 100 % conserved in mouse/rat, 91 % in golden hamster, 83 % in rabbit and pig, and 75 % in human and canine UCP1. It has no significant homology with UCP2-5.

Purification: Affinity chromatography purified

Immunogen: UCP1 antibody was raised in rabbit using a 12 amino acid peptide from mouse/rat UCP1 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS to a concentration of 1 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 0.1 % BSA.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP1

Background: Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs).

Pathways: Proton Transport, Brown Fat Cell Differentiation