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UCP1 antibody (AA 179-296)

UCP1 Reactivity: Mouse WB, IHC, IP, IF/ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2905048
  • Target See all UCP1 Antibodies
    UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))
    Binding Specificity
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 179-296
    Reactivity
    • 28
    • 19
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 35
    • 4
    • 1
    Rabbit
    Clonality
    • 37
    • 3
    Polyclonal
    Conjugate
    • 20
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This UCP1 antibody is un-conjugated
    Application
    • 29
    • 17
    • 14
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    UCP1 (Pro179-Leu296)
    Top Product
    Discover our top product UCP1 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Validation #103357 (Western Blotting)
    'Independent Validation' Badge
    by
    Mivechi lab, Georgia Cancer Center, Augusta University
    No.
    #103357
    Date
    10/03/2018
    Antigen
    UCP1
    Lot Number
    AB1808U769
    Method validated
    Western Blotting
    Positive Control

    Cold exposed mouse brown adipose tissue (BAT)

    Negative Control

    Mouse liver tissue

    Notes

    Passed. ABIN3056733 can detect mouse UCP1 protein in immunoblotting analysis.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3056733
    Secondary Antibody
    donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152)
    Full Protocol
    • Homogenize mouse brown fat tissue in 2ml mitochondria isolation buffer (70mM sucrose, 210mM mannitol, 5mM HEPES, 1mM EGTA, pH7.2, and 0.5% BSA).
    • Centrifuge the homogenates for 10min at 800g at 4°C.
    • Centrifuge the supernatant again at 8000g for 10min at 4°C.
    • Lyse the resulting mitochondria fraction in RIPA buffer containing protease inhibitor cocktail (Pierce Protease Inhibitor, A32965, lot TA2492391).
    • Determined protein concentration with Pierce BCA Protein Assay Kit (Pierce, 23225, lot OA180049).
    • Separate 50µg of total protein per lane on a denaturing 12% SDS-PAGE gel (Bio-Rad, 1610156, lot 64144809) in a Mini Protean Tetra Cell for 2h at 80V.
    • Transfer proteins onto Immobilon-P PVDF Membrane (Millipore, IPVH00010) using Mini Trans Blot Cell system (Bio-Rad, 1703930).
    • Block membrane with 5% milk in PBS-T for 1h at RT.
    • Incubate membrane with primary rabbit anti-UCP1 antibody (antibodies-online, ABIN3056733, lot AB1808U769) diluted to 1µg/ml ON at 4°C.
    • Wash membrane 3x for 10min with PBS-T.
    • Incubate membrane with secondary donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152) for 1h at RT.
    • Wash membrane 3x for 10min with PBS-T.
    • Incubate membrane Pierce ECL Western Blotting Substrate working solution (Thermo Scientific, 1859701, lot TB263310) for 1min at RT. Expose membrane to x-ray film for different amount of time.
    Experimental Notes

    The UCP1 antibody ABIN3056733 reveals a specific protein band in positive control samples (cold exposed mouse brown fat tissue), but not in negative control samples (liver tissue).

  • Format
    Liquid
    Concentration
    500 μg/mL
    Buffer
    0.01M PBS,  pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4 °C for frequent use. Aliquot and store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
  • Target
    UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))
    Alternative Name
    Uncoupling Protein 1, Mitochondrial (UCP1 Products)
    Synonyms
    ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1 antibody, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1 antibody, ATPUMP1 antibody, ATUCP1 antibody, UCP antibody, UCP1 antibody, UNCOUPLING PROTEIN 1 antibody, plant uncoupling mitochondrial protein 1 antibody, ucp antibody, slc25a7 antibody, SLC25A7 antibody, AI385626 antibody, Slc25a7 antibody, Ucp antibody, Ucpa antibody, Uncp antibody, plant uncoupling mitochondrial protein 1 antibody, uncoupling protein 1 antibody, uncoupling protein 1 L homeolog antibody, uncoupling protein 1 (mitochondrial, proton carrier) antibody, PUMP1 antibody, UCP1 antibody, ucp1.L antibody, ucp1 antibody, Ucp1 antibody
    UniProt
    P12242
    Pathways
    Proton Transport, Brown Fat Cell Differentiation
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