UCP1 antibody (Uncoupling Protein 1 (Mitochondrial, Proton Carrier)) (AA 179-296)

Details for Product anti-UCP1 Antibody No. ABIN2905048, Supplier: Log in to see
Antigen
  • ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1
  • ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1
  • ATPUMP1
  • ATUCP1
  • UCP
  • UCP1
  • UNCOUPLING PROTEIN 1
  • plant uncoupling mitochondrial protein 1
  • ucp
  • slc25a7
  • SLC25A7
  • AI385626
  • Slc25a7
  • Ucp
  • Ucpa
  • Uncp
  • plant uncoupling mitochondrial protein 1
  • uncoupling protein 1
  • uncoupling protein 1 L homeolog
  • uncoupling protein 1 (mitochondrial, proton carrier)
  • PUMP1
  • UCP1
  • ucp1.L
  • ucp1
  • Ucp1
Alternatives
anti-Human UCP1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 179-296
31
12
11
6
4
3
3
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
107
56
35
2
2
2
1
1
1
1
Host
Rabbit
80
39
19
Clonality
Polyclonal
Conjugate
This UCP1 antibody is un-conjugated
11
8
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
117
72
54
10
9
6
5
4
1
1
1
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Antigen UCP1
Lot Number AB1808U769
Method validated Western Blotting
Positive Control

Cold exposed mouse brown adipose tissue (BAT)

Negative Control

Mouse liver tissue

Notes

Passed. ABIN3056733 can detect mouse UCP1 protein in immunoblotting analysis.

Primary Antibody ABIN3056733
Secondary Antibody donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152)
Protocol
  • Homogenize mouse brown fat tissue in 2ml mitochondria isolation buffer (70mM sucrose, 210mM mannitol, 5mM HEPES, 1mM EGTA, pH7.2, and 0.5% BSA).
  • Centrifuge the homogenates for 10min at 800g at 4°C.
  • Centrifuge the supernatant again at 8000g for 10min at 4°C.
  • Lyse the resulting mitochondria fraction in RIPA buffer containing protease inhibitor cocktail (Pierce Protease Inhibitor, A32965, lot TA2492391).
  • Determined protein concentration with Pierce BCA Protein Assay Kit (Pierce, 23225, lot OA180049).
  • Separate 50µg of total protein per lane on a denaturing 12% SDS-PAGE gel (Bio-Rad, 1610156, lot 64144809) in a Mini Protean Tetra Cell for 2h at 80V.
  • Transfer proteins onto Immobilon-P PVDF Membrane (Millipore, IPVH00010) using Mini Trans Blot Cell system (Bio-Rad, 1703930).
  • Block membrane with 5% milk in PBS-T for 1h at RT.
  • Incubate membrane with primary rabbit anti-UCP1 antibody (antibodies-online, ABIN3056733, lot AB1808U769) diluted to 1µg/ml ON at 4°C.
  • Wash membrane 3x for 10min with PBS-T.
  • Incubate membrane with secondary donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152) for 1h at RT.
  • Wash membrane 3x for 10min with PBS-T.
  • Incubate membrane Pierce ECL Western Blotting Substrate working solution (Thermo Scientific, 1859701, lot TB263310) for 1min at RT. Expose membrane to x-ray film for different amount of time.
Experimental Notes

The UCP1 antibody ABIN3056733 reveals a specific protein band in positive control samples (cold exposed mouse brown fat tissue), but not in negative control samples (liver tissue).

Validation Images
Western Blotting validation image for anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 179-296) antibody (ABIN3056733) Lysates from mitochondrial fraction isolated from mouse liver tissue (negative contro...
Immunogen UCP1 (Pro179-Leu296)
Purification Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Plasmids, Primers & others Plasmids, Primers & others UCP1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Uncoupling Protein 1, Mitochondrial (UCP1 Antibody Abstract)
UniProt P12242
Pathways Proton Transport, Brown Fat Cell Differentiation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 500 μg/mL
Buffer 0.01M PBS,  pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4 °C for frequent use. Aliquot and store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 179-296) antibody (ABIN2905048) Western blot analysis of recombinant Mouse UCP1.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 179-296) antibody (ABIN2905048) IHC-P analysis of Mouse Tissue, with DAB staining.
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